Production of polyketides in bacteria and yeast

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/00 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/70 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01) C12P 7/42 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2274087

Hybrid and novel polyketide synthases and polyketides are produced by use of a multiple vector system. The combinatorial possibilities offered by placing the various catalytic activities of PKS systems on separate vectors permits the construction of improved libraries of PKS and polyketides. In addition, polyketides can be produced in hosts that ordinarily do not produce polyketides by supplying, along with an expression system for the desired PKS, an expression system for holo ACP synthase.

L'invention porte sur de nouvelles synthases de polycétides (PKS) hybrides et de nouveaux polycétides hybrides produits à l'aide d'un système de vecteurs multiples. Les possibilités combinatoires offertes lorsqu'on place les différentes activités catalytiques des systèmes de PKS sur des vecteurs séparés permettent la constitution de bibliothèques améliorées de PKS et de polycétides. De plus, les polycétides peuvent être produits dans des hôtes ne produisant pas ordinairement de polycétides, en utilisant parallèlement au système d'expression des PKS désirés un système d'expression de l'holosynthase PTA.

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