Production of poxviruses with adherent or non adherent avian...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/00 (2006.01) C12N 5/0735 (2010.01) A61K 39/285 (2006.01) A61P 31/20 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2531565

The present invention relates to a method for replicating poxviruses such as vaccinia virus comprising the steps of inoculating avian embryonic stem cells with viral particles and culturing said cells in a basal medium until cells lysis occurs and newly produced viral particles are released in said medium.

La présente invention concerne un procédé permettant la réplication de poxvirus, par exemple le virus de la vaccine, et comportant les étapes consistant à inoculer des particules virales à des cellules souches embryonnaires aviaires et à mettre en culture lesdites cellules dans un milieu basique jusqu'à ce que la lyse des cellules se produise et que des particules virales nouvellement produites soient libérées dans ledit milieu.

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