Production of protein in insect larvae using a...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/90 (2006.01) A01K 67/033 (2006.01) C07K 14/47 (2006.01) C07K 14/61 (2006.01) C12N 9/22 (2006.01) C12N 9/24 (2006.01) C12N 15/54 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2387991

The invention relates to a method for producing a protein of interest, comprising transforming an insect with a non-viral expression system capable of expressing the protein of interest in the larvae of the insect; breeding the insect to produce larvae; culturing the larvae; and isolating the protein of interest from the larvae. The transformation is advantageously performed by means of a transposon vector.

L'invention concerne un procédé de production de protéine d'intérêt. Ce procédé consiste à transformer un insecte avec un système d'expression non viral capable d'exprimer la protéine d'intérêt dans sa larve, à accoupler l'insecte de manière à ce qu'il produise des larves, à cultiver les larves, et à isoler la protéine d'intérêt de la larve. La transformation est avantageusement effectuée au moyen d'un vecteur transposon.

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