Production of proteins

Details Production of proteins Production of proteins

C - Chemistry, Metallurgy

12

N

C12N 15/79 (2006.01) C07K 14/415 (2006.01) C07K 14/425 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2589164

A method for forming a fusion protein that is expressed as a recombinant protein body-like assembly in host eukaryotic cells and organisms other than higher plants as host systems is disclosed. More particularly, peptides and proteins are fused to protein sequences that mediate the induction of recombinant protein body-like assembly (RPBLA) formation, are stably expressed and accumulated in these host cells after transformation with an appropriate vector. Methods for preparing the fusion protein are also disclosed.

L'invention concerne une méthode de formation d'une protéine de fusion exprimée comme un ensemble de type protéine de recombinaison dans des cellules eucaryotes hôtes et des organismes, autres que des plantes supérieures, constituant des systèmes hôtes. L'invention concerne, plus particulièrement, des peptides et des protéines fondus en séquences protéiques médiant l'induction de la formation d'un ensemble de type protéine de recombinaison (RPBLA), exprimés de manière stable et accumulés dans lesdites cellules hôtes après leur transformation à l'aide d'un vecteur approprié. L'invention concerne également des méthodes de préparation de ladite protéine de fusion.

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