Production of proteins by autoproteolytic cleavage

Details Production of proteins by autoproteolytic cleavage Production of proteins by autoproteolytic cleavage

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C07K 14/54 (2006.01) C07K 14/56 (2006.01) C12N 1/21 (2006.01) C12N 9/50 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2380302

The present invention relates to a process for the production of a desired heterologous polypeptide with a clearly defined homogeneous N-terminus in a bacterial host cell, wherein the desired heterologous polypeptide is autoproteolytically cleaved from an initially expressed fusion protein which comprises a polypeptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide by the Npro autoproteolytic activity.

La présente invention concerne un processus de production d'un polypeptide hétérologue avec une terminaison N homogène et clairement définie dans une cellule hôte bactérienne. Ce polypeptide hétérologue recherché est coupé de façon autoprotéolytique d'une protéine hybride initialement exprimée qui comprend un polypeptide avec l'activité autoprotéolytique d'une autoprotéase N?pro¿ d'un pestivirus et ce polypeptide hétérologue obtenu par l'activité N?pro¿ autoprotéolytique.

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