Production of proteins in plant seeds

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2305628

Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein. Also disclosed are improved methods for transforming plants, including cereals such as barley, corn or wheat.

L'invention concerne des procédés de fabrication de protéines dans des graines de plantes. Un promoteur propre à la graine dirige l'expression de la protéine; de préférence, la protéine est exprimée sous la forme d'un polypeptide de fusion comportant un peptide signal qui permet à la protéine de s'accumuler dans un compartiment intracellulaire et protège ainsi ladite protéine. L'invention concerne également des procédés améliorés permettant de transformer des plantes, y compris des céréales telles que l'orge, le maïs et le blé.

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