Production of recombinant human hemoglobin using pichia yeast

Details Production of recombinant human hemoglobin using pichia yeast Production of recombinant human hemoglobin using pichia yeast

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C07K 14/805 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2610098

The present invention provides a production method of a functional recombinant human hemoglobin, which comprises transforming a yeast of the genus Pichia with an expression vector containing a DNA encoding a human hemoglobin .alpha.-chain (Hb.alpha.), which is under regulation of a promoter functional in a yeast of the genus Pichia, and an expression vector containing a DNA encoding a human hemoglobin .beta.-chain (Hb.beta.), which is under regulation of a promoter functional in a yeast of the genus Pichia, culturing the yeast in a medium, and recovering the human hemoglobin from the obtained culture. The present invention also provides an expression vector containing AOX1 promoter, DNA encoding Hb.beta., terminator functional in a yeast of the genus Pichia, AOX1 promoter, DNA encoding Hb.alpha. and terminator functional in the yeast of the genus Pichia in this order from the upstream side in transcriptional direction.

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