Production of recombinant peptides as natural hydrophobic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/67 (2006.01) A61K 31/70 (2006.01) A61K 35/74 (2006.01) A61K 48/00 (2006.01) C07K 14/135 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 15/45 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2204511

A method for secreting a biologically active recombinant peptide as an analogue of a natural peptide having at least one hydrophobic region, by culturing cells transformed by a nucleic acid construct comprising elements for the expression and secretion of said peptide by the cell, and a sequence coding for a peptide with an amino acid sequence that differs from the natural peptide sequence in that it has at least one modification in a non-transmembrane hydrophobic region of the peptide; and by recovering the peptide and/or cells carrying said recombinant peptide. The resulting recombinant peptide, a corresponding DNA sequence, and a bacterium containing same, are also disclosed.

La présente invention concerne un procédé de sécrétion d'un peptide recombinant biologiquement actif, analogue d'un peptide naturel présentant au moins une région hydrophobe, caractérisé en ce qu'on cultive des cellules transformées par une construction d'acides nucléiques comportant des éléments assurant l'expression et la sécrétion dudit peptide par la cellule, et une séquence codant pour un peptide dont la séquence en acides aminés diffère de la séquence du peptide naturel par au moins une modification dans une région hydrophobe non transmembranaire du peptide, et en ce que l'on récupère le peptide et/ou les cellules portant ledit peptide recombinant. L'invention concerne également un peptide recombinant susceptible d'être ainsi obtenu, une séquence d'ADN correspondante et une bactérie la contenant.

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