C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A01K 67/027 (2006.01) A23C 9/00 (2006.01) C07K 14/00 (2006.01) C12N 9/00 (2006.01)
Patent
CA 2351200
A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one ex- pression regulation sequence, a secretory DNA sequence encoding a secretory signal which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptides. A method for producing transgenic bovine species comprises introducing the above transgene into an embryonal target cell of a bovine species, transplan- ting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female off, spring which is capable of producing the recombinant polypeptide in its milk. The invention also includes transgenic bovine spe- cies capable of producing recombinant polypeptides in transgenic milk as well as the milk from such transgenic bovine species and food formulations containing one or more recombinant polypeptide. A method for producing transgenic non-human mam- mals having a desirable phenotype comprises first methylating a transgene followed by introduction into fertilized oocytes. The oocytes are then cultured to form preimplantation embryos. Thereafter, at least one cell is removed from each of the pre-implan- tation embryos and the DNA digested with restriction endonuclease capable of cleaving the methylated transgene but incapable cleaving the unmethylated form of the transgene.
Deboer Herman A.
Heyneker Herbert L.
Lee Sang He
Platenburg Gerard
Strijker Rein
Gene Pharming Europe Bv
Smart & Biggar
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