Production of recombinant proteins using herpes virus...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C07K 14/52 (2006.01) C07K 14/61 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 9/10 (2006.01) C12N 9/72 (2006.01) C12N 15/12 (2006.01) C12N 15/38 (2006.01) C12N 15/54 (2006.01) C12N 15/58 (2006.01) C12N 15/67 (2006.01) C12N 15/869 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2092645

07-21(690)A Abstract of the Disclosure Stable cell lines are produced to express high levels of a gene product of interest using VP16, a herpes simplex virus transactivator, and a promoter from herpes simplex virus which is a target for VP16. The transactivator and promoter are introduced to a cell line separately using antibiotic resistance genes as selectable markers on separate vectors. Inventors: Paul Hippenmeyer Maureen Highkin

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