Production of reprogrammed cells with restored potential

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01)

Patent

CA 2617611

A method for treating cells and/or nuclear transfer units and/or stem cells in culture with such compounds, individually or in combinations, is described. The method results in a globally hypomethylated genome and a restoration of cell differentiation and/or developmental potential, or potentiality. In addition, a method for the in vitro production of reprogrammed cells which have had differentiation potential (totipotential, pluripotential, or multipotential) restored by demethylating the genome is described.

L'invention concerne un procédé de traitement de cellules et/ou d'unités de transfert nucléaire et/ou de cellules souches en culture avec des composés utilisés seuls ou combinés. Le procédé produit un génome globalement hypométhylé et un rétablissement de la différenciation cellulaire et/ou du pouvoir de développement ou de la potentialité. L'invention concerne également un procédé de production in vitro de cellules reprogrammées dont le pouvoir de différenciation (totipotence, pluripotence ou multipotence) a été rétabli par déméthylation du génome.

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