Production of secondary metabolites using capillary membranes

Details Production of secondary metabolites using capillary membranes Production of secondary metabolites using capillary membranes

C - Chemistry, Metallurgy

12

M

C12M 1/12 (2006.01) C12M 1/00 (2006.01) C12N 15/09 (2006.01) C12P 1/00 (2006.01) C12P 1/02 (2006.01) C12P 1/04 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2613237

The invention provides methods of producing secondary metabolites and under recombinant products oxygen-limited or anaerobic culture conditions. The methods include providing a porous substrate having a first side and a second side and which has a biofilm of microorganisms attached to the first side thereof, and causing a nutrient solution to flow through the biofilm and the substrate in a direction from the first side thereof to the second side thereof under oxygen-limited or anaerobic culture conditions. The microorganisms include Clostridium sp., Bacillus sp., Pseudomonas sp. Vibrio sp., Serratia sp., Rhodopseudomonas sp., Anaeromyxobacter sp., Desulphovibrio sp., and Candida sp., Lactococcus lactis, Escherichia coli, Bacillus subtillus, Pichia sp., Candida sp., Hansenula polymorpha and Sacharomyces cerevisiae.

L'invention concerne des procédés permettant de produire des métabolites secondaires et/ou des produits recombinés dans des conditions de culture pauvres en oxygène ou anaérobies. Ces procédés consistent à prendre un substrat poreux qui présente un premier et un second côté, et qui comprend un film biologique de micro-organismes fixé sur le premier côté, et à faire circuler une solution nutritive à travers le film biologique et le substrat, du premier côté en direction du second côté, dans des conditions de culture pauvres en oxygène ou anaérobies. Les micro-organismes utilisés comprennent Clostridium sp., Bacillus sp., Pseudomonas sp. Vibrio sp., Serratia sp., Rhodopseudomonas sp., Anaeromyxobacter sp., Desulphovibrio sp., et Candida sp., Lactococcus lactis, Escherichia coli, Bacillus subtillus, Pichia sp., Candida sp., Hansenula polymorpha et Sacharomyces cerevisiae.

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