Production of self-compatible brassica hybrids using a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01)

Patent

CA 2279496

This invention relates to cells of a self-compatible hybrid plant, the parents of which are a homozygous self-incompatible female parent, and a homozygous male parent, and the nuclear genome of which contains a vector comprising a DNA sequence which, when expressed in a plant cell, imparts self- incompatibility to the plant, and a promoter capable of directing the expression of the DNA sequence in the cell. The invention includes the vector, a plant comprising the cells, a plant transformed with the vector, the seed of such plants, and a method for conferring the self-compatible phenotype on progeny of the self-incompatible female parent and self-compatible male parent. The invention comprises an improved self-pollination control system for hybrid seed production or breeding which, by using a knockout transgene to eliminate the self-incompatibility phenotype in hybrids, can result in increased yields.

L'invention concerne des cellules d'une plante hybride auto-compatible, dont les parents sont un parent femelle homozygote auto-incompatible et un parent mâle homozygote et dont le génome nucléaire contient un vecteur comprenant une séquence d'ADN qui, lorsqu'elle est exprimée dans une cellule, confère un auto-incompatibilité à la plante, et un promoteur capable de diriger l'expression de la séquence d'ADN dans la cellule. L'invention porte également sur le vecteur, sur une plante comprenant les cellules, sur une plante transformée à l'aide dudit vecteur, sur les graines desdites plantes et sur un procédé permettant de conférer le phénotype auto-compatible à la progéniture du parent femelle auto-incompatible et du parent mâle auto-compatible. Elle se rapporte enfin à un système de régulation d'auto-pollinisation pour la production ou la sélection de graines hybrides, qui, grâce à un transgène de suppression permettant d'éliminer le phénotype d'auto-incompatibilité dans des hybrides, peut augmenter le rendement.

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