Production of soluble recombinant trypsinogen analogs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/76 (2006.01) C12N 15/11 (2006.01) C12N 15/57 (2006.01) C12N 15/62 (2006.01) C12N 15/79 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2343966

The present invention relates to recombinantly produced trypsin and trypsinogen analogs, including nucleic acids which encode trypsin and trypsinogen analogs. The trypsinogen analogs of the present invention contain modifications of the trypsinogen leader sequence such that the trypsinogen cannot be cleaved by trypsin or trypsin-like enzymes. The present invention also includes processes for producing recombinant trypsinogen analogs which can subsequently be activated.

La présente invention porte sur des analogues de trypsine et de trypsinogène obtenus par recombinaison et comprenant des acides nucléiques les codant. Les analogues de trypsinogène de cette invention comportent des modifications de la séquence de tête du trypsinogène de sorte que le trypsinogène ne puisse être clivé par la trypsine ou les enzymes analogues à la trypsine. Cette invention porte également sur des procédés de production de ces analogues de trypsinogène de recombinaison qui peuvent être ultérieurement activés.

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