Production of syringyl lignin in gymnosperms

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2314883

The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm. In addition, genetic sequences which code for gymnosperm lignin anti-sense mRNA may be incorporated into the gymnosperm genome in order to suppress the formation of the less preferred forms of lignin in the gymnosperm such as guaiacyl lignin.

L'invention concerne un procédé de production de lignine constituée de syringyle chez les gymnospermes. Cette production est accomplie par transformation génétique d'un génome de gymnosperme, qui normalement ne contient pas de gènes codant pour les enzymes nécessaires à la production de lignine constituée de syringyle, au moyen d'un ADN codant pour les enzymes d'angiospermes associés à la production de lignine constituée de syringyle. L'expression de l'ADN introduit est induite dans le gymnosperme au moyen de régions promotrices d'hôtes. En outre, les séquences génétiques qui codent pour l'ARN messager antisens de la lignine du gymnosperme peuvent être incorporées dans le génome du gymnosperme afin d'empêcher la formation dans le gymnosperme de formes moins préférées de lignine, telles que la lignine constituée de guaiacyle.

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