Production of tailored oils in heterotrophic microorganisms

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/13 (2006.01) A23D 9/00 (2006.01) A23K 1/16 (2006.01) C10L 1/04 (2006.01) C10L 1/06 (2006.01) C11C 3/00 (2006.01) C11C 3/04 (2006.01) C11C 3/12 (2006.01) C12N 1/12 (2006.01) C12N 15/00 (2006.01) C12N 15/52 (2006.01) C12N 15/79 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2745129

Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in Prototheca are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Prototheca cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a fatty acyl-ACP thioesterase, a fatty acyl- CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel.

La présente invention concerne des procédés et des compositions pour la production d'huiles, de combustibles, de substances oléochimiques, et d'autres composés dans Prototheca, comprenant des micro-organismes contenant de l'huile et des procédés de culture à faible coût de tels micro-organismes. Des cellules de Prototheca contenant des gènes exogènes codant pour, par exemple, une lipase, un transporteur de saccharose, une saccharose invertase, une fructokinase, une enzyme de dégradation de polysaccharide, une (acyle gras)-ACP thioestérase, une (acyle gras)-CoA/aldéhyde réductase, une (acyle gras)-CoA réductase, une aldéhyde gras réductase, une aldéhyde gras décarbonylase, et/ou une protéine de transport d'acyle, sont utiles dans la fabrication de combustibles de transport tels qu'un carburant diesel renouvelable, un biodiesel, et un carburéacteur renouvelable.

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