Production of water stress or salt stress tolerant...

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2234168

The present invention is directed to a method of producing a cereal plant cell or protoplast useful for regeneration of a water stress or salt stress tolerant cereal plant by transforming the cereal plant cell or protoplast with a nucleic acid encoding a late embryogenesis abundant protein. A transgenic cereal plant or cereal plant cell or protoplast transformed with a nucleic acid encoding a late embryogenesis abundant protein is also provided. An LEA protein gene, HVA1, from barley (Hordeum vulgare L.) was transformed into rice (Oryza sativa L.) plants. The resulting transgenic rice plants accumulate the HVA1 protein in both leaves and roots. Transgenic rice plants showed significantly increased tolerance to water stress (drought) and salt stress.

Un procédé permet de produire une cellule ou un protoplaste de plante céréalière, utile pour régénérer une plante céréalière tolérante au stress hydrique ou salin, en transformant cette cellule ou ce protoplaste de plante céréalière avec un acide nucléique codant une protéine abondante en fin d'embryogenèse. On décrit aussi une plante céréalière transgénique ou une cellule ou un protoplaste de plante céréalière transformé avec un acide nucléique codant une protéine abondante en fin d'embryogenèse. On a transformé un gène de protéine abondante en fin d'embryogenèse provenant de l'orge (Hordeum vulgare L.), HVA1, en plants de riz (Oryza sativa L.). Les plants de riz transgéniques résultants accumulent la protéine HVA1 tant dans les feuilles que dans les racines. Ces plants de riz transgéniques ont démontré une tolérance significativement accrue aux stress hydrique (sécheresse) et salin.

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