C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 1/19 (2006.01) C12N 9/16 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2421592
The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.
Geipel Frank
Hoelke Werner
Kirschbaum Thomas
Mueller Rainer
Thalhofer Johann-Peter
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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