Production of weakly active or inactive mutants of alkaline...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/55 (2006.01) C12N 1/19 (2006.01) C12N 9/16 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2421592

The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.

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