Production process of recombinant placental growth factor

Details Production process of recombinant placental growth factor Production process of recombinant placental growth factor

C - Chemistry, Metallurgy

07

K

C07K 14/475 (2006.01) C07K 14/515 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2475235

Process for extracting and purifying the recombinant Placental Growth Factor (PLGF) expressed in inducible prokaryotic expression systems comprising the following steps: I) fermentation of the bacterial cells, II) extraction and purification of the inclusion bodies, III) renaturation of the expressed protein, IV) ion-exchange chromatography, V) reverse-phase chromatography.

L'invention concerne un procédé d'extraction et de purification du facteur de croissance placentaire recombinant (PLGF) exprimé dans des systèmes d'expression procaryotes inductibles comprenant les étapes consistant à: (I) faire fermenter des cellules bactériennes, (II) extraire et purifier les corps d'inclusion, (III) renaturer la protéine exprimée, (IV) effectuer une chromatographie d'échange ionique, (V) effectuer une chromatographie de phase inverse.

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