C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/475 (2006.01) C07K 14/515 (2006.01) C12N 15/12 (2006.01)
Patent
CA 2475235
Process for extracting and purifying the recombinant Placental Growth Factor (PLGF) expressed in inducible prokaryotic expression systems comprising the following steps: I) fermentation of the bacterial cells, II) extraction and purification of the inclusion bodies, III) renaturation of the expressed protein, IV) ion-exchange chromatography, V) reverse-phase chromatography.
L'invention concerne un procédé d'extraction et de purification du facteur de croissance placentaire recombinant (PLGF) exprimé dans des systèmes d'expression procaryotes inductibles comprenant les étapes consistant à: (I) faire fermenter des cellules bactériennes, (II) extraire et purifier les corps d'inclusion, (III) renaturer la protéine exprimée, (IV) effectuer une chromatographie d'échange ionique, (V) effectuer une chromatographie de phase inverse.
Battisti Mauro
Conti Ettore
Maglione Domenico
Salvia Giuseppe
Tucci Marina
Fetherstonhaugh & Co.
Geymonat S.p.a.
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