Products and processes for multiplex nucleic acid...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2742272

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the oligonucleotides include distinguishable labels and a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing and detecting the distinguishable label, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the distinguishable label.

La présente invention concerne des produits et des procédés pour détecter la présence ou l'absence d'acides nucléiques cibles multiples. Certains procédés comprennent l'amplification des acides nucléiques cibles, ou une portion de ceux-ci ; l'extension d'oligonucléotides qui s'hybrident spécifiquement avec les amplicons, où les oligonucléotides comprennent des marqueurs distinctifs et un agent de capture ; la capture des oligonucléotides étendus sur une phase solide par l'intermédiaire de l'agent de capture ; la libération et la détection du marqueur distinctif, et ainsi la détermination de la présence ou l'absence de chaque acide nucléique cible par la présence ou l'absence du marqueur distinctif.

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