C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0775 (2010.01) C12N 5/077 (2010.01) C12N 5/0797 (2010.01) A61K 35/28 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2444959
Described is a method of expanding human progenitor cells by suspension culturing under non-static conditions. The culturing method provides a three- dimensional space for the rapid expansion of desirable progenitors. By this method, a new compartment of multipotential progenitor cells has been identified, which give rise under differentiation conditions to progeny including osteoblasts, chondrocytes, myoblasts, adipocytes, and other non- hematopoietic cell types. Their use in cell and tissue-based engineering is described.
L'invention concerne une méthode d'expansion de cellules souches humaines par culture en suspension dans des conditions non statiques. La méthode de culture fournit un espace tridimensionnel conçu pour permettre l'expansion rapide des cellules souches souhaitées. Cette méthode a permis l'identification d'une nouvelle classe de cellules souches multipotentielles qui, lorsqu'elles ont soumises à des conditions de différentiation, donnent des cellules descendantes parmi lesquelles figurent des ostéoblastes, des chondrocytes, des adipocytes et d'autres espèces de cellules non hématopoïétiques. L'invention concerne en outre leur utilisation en génie cellulaire et tissulaire.
Baksh Dolores
Davies John E.
Zandstra Peter
Baksh Dolores
Davies John E.
Gastle And Associates
Zandstra Peter
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