Prokaryotic xylose isomerase for the construction of xylose...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 9/92 (2006.01) C12N 15/81 (2006.01) C12P 7/10 (2006.01) C12P 19/24 (2006.01)

Patent

CA 2728682

The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol. The present invention also relates to methods for the production of bioethanol, and to methods for the production of further metabolization products, comprising the metabolization of media containing xylose.

L'invention concerne l'utilisation de molécules d'acide nucléique codant pour une xylose-isomérase (XI) bactérienne qui provient, de préférence, de Clostridium phytofermentans, pour la réaction/métabolisme, en particulier la fermentation, avec des micro-organismes recombinants d'un biomatériau contenant du xylose, et en particulier la production de bioalcools, notamment de bioéthanol, au moyen de levures fermentant le xylose. L'invention concerne en outre des cellules, en particulier des cellules eucaryotes qui sont transformées avec une structure d'expression d'acide nucléique codant une xylose-isomérase (XI), l'expression de la structure d'expression d'acide nucléique conférant aux cellules la capacité d'isomériser le xylose directement en xylulose. Ces cellules sont de préférence utilisées pour la réaction/métabolisme, en particulier la fermentation, d'un biomatériau contenant du xylose, et en particulier pour la production de bioalcools, notamment de bioéthanol. L'invention concerne en outre un procédé de production de bioéthanol, et un procédé de production d'autres produits du métabolisme, comprenant le métabolisme de milieux qui renferment du xylose.

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