C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2062963
2062963 9118115 PCTABS00008 This invention discloses a scheme for producing nucleic acid end products that are functionally or exactly identical to the starting products, thereby resulting in exponential amplification of a desired nucleic acide sequence. Specifically, sequences are cycled between RNA and DNA forms using the following basic steps: (1) a T7 RNA polymerase promoter is ligated onto a single-stranded DNA template; (2) T7 RNA polymerase makes many copies of RNA: (3) a complementary DNA is made from the RNA by extension of a primer by reverse transcriptase; and (4) the RNA template is removed by ribonuclease H. This amplification method is useful for purposes such as genetic research and diagnostic assays.
Berninger Mark S.
Rashtchian Ayoub
Schuster David M.
Berninger Mark S.
Gowling Lafleur Henderson Llp
Life Technologies Inc.
Rashtchian Ayoub
Schuster David M.
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