Promoter sequence obtained from rice and methods of use

Details Promoter sequence obtained from rice and methods of use Promoter sequence obtained from rice and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2633517

Methods are provided by which Oryza saliva plants and seeds thereof may be modified to express a coding region of interest using a promoter sequence operatively linked to the coding region. The promoter sequence is an isolated Oryza sativa antiquitin (OsAnt1) promoter sequence including SEQ ID NO: 1. The coding region of interest may encode a nitrogen utilization protein, suitably alanine aminotransferase. Methods to develop Oryza sativa plants that have increased biomass and seed yield are also presented. Furthermore, Oryza saliva plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

L'invention porte sur des procédés qui permettent de modifier des plantes d'Oryza Sativa et leurs graines pour exprimer une région de codage d'intérêt en utilisant une séquence promotrice liée de manière fonctionnelle à la région de codage. La séquence promotrice est une séquence promotrice (OsAntl) d'antiquitined'Oryza Sativa renfermant la séquence SEQ ID NO: 1. La région de codage d'intérêt peut coder une protéine d'utilisation de l'azote, de préférence l'alanine aminotransférase. L'invention porte également sur des procédés permettant de développer les plantes d'Oryza Sativa qui ont augmenté la biomasse et le rendement en grain. Le procédé permet également de produire des plantes d'Oryza Sativa qui conservent un rendement désiré tout en réduisant le besoin en niveaux élevés d'application d'azote.

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