Promoter, vector having the improved promoter, plasmid...

Details Promoter, vector having the improved promoter, plasmid... Promoter, vector having the improved promoter, plasmid...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01)

Patent

CA 1301678

Abstract of the Disclosure: An improved promoter of E. coli, in which (a) the -35 region in the promoter shows the following base sequence 5'-TTGACAX-3' AACTGTY wherein the XY base pair represents a base pair of either TA, GC, AT or CG, and/or the -10 region in the promoter shows the follow- ing base sequence 5'-TATAAT-3' ATATTA (b) an intermediate region between the -35 region and the -10 region in the promoter consists of 15 to 17 base pairs and shows such a base sequence in which the proportion of base pairs CG and GC in the entire base pairs in this region is at least 60 % and a vector having the improved promoter.

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