C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/86 (2006.01) A61K 39/12 (2006.01) C12N 7/00 (2006.01) C12N 15/40 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2689588
The present invention provides an isolated RNA molecule comprising: a) an alphavirus 5' replication recognition sequence, wherein at least one initiation codon has been removed from the 5' replication recognition sequence; b) a nucleotide sequence encoding an alphavirus structural protein; and c) an alphavirus 3' replication recognition sequence, with the proviso that the RNA molecule does not contain a promoter that directs transcription of the nucleotide sequence of (b), and wherein the alphavirus 5' and 3' replication recognition sequences of (a) and (c) direct replication of the RNA molecule in the presence of alphavirus nonstructural proteins.
La présente invention concerne une molécule d'ARN isolée comprenant: a) une séquence de reconnaissance de réplication 5' d'alphavirus, au moins un codon initiateur ayant été retiré de la séquence de reconnaissance de réplication 5'; b) une séquence nucléotidique codant pour une protéine structurale d'alphavirus; et c) une séquence de reconnaissance de réplication 3' d'alphavirus, à condition que la molécule d'ARN ne contienne pas un promoteur qui dirige la transcription de la séquence nucléotidique de (b). En outre, les séquences de reconnaissance de réplication 5' et 3' d'alphavirus (a) et (c) dirigent la réplication de la molécule d'ARN en présence de protéines non structurales d'alphavirus.
Kamrud Kurt I.
Maughan Maureen
Smith Jonathan F.
Alphavax Inc.
Smart & Biggar
LandOfFree
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