C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01)
Patent
CA 2422480
Late embryogenesis abundant (Lea) proteins accumulate in maturing seeds after many of the storage compounds have been synthesized, and they are considered relevant to maturation. We report here the molecular organization and expression of BnLea3-1, a novel Group 3 Lea gene from Brassica napus. BnLea3-1 contains a coding region of 798 bp, sharing 84.4% homology at the amino acid level with Lea76 of B. napus. Two tandem 11-mer repeats are truncated from the coding region of BnLea3-l, compared to the 13 conserved 11-mer repeats of Lea76. Substitutions of consensus residues are found at various positions within the 11-mer repeats. A 1561 by 5' flanking promoter fragment of BnLea3-1 fused to E. coli .beta.3- glucurondiase (GUS) coding region conferred seed-specific GUS expression in stable transgenics of B. napus, tobacco and in transiently-transformed pea. A -137 by minimal promoter preceding the first transcription start site, identified through progressive deletions from the upstream, was sufficient for basal GUS expression in the seeds arid in leaves treated with ABA. Deletion studies indicate the presence of enhancing elements located between -137 by to -742 by and suppressing elements located between - 742 and -1561 bp. Bnlea3-1 expression in seeds precedes that of Lea76. Unlike other Group 3 Lea members including HVA1 and Dc3, BnLea3-1 is active in seeds and responsive weakly in vegetative tissues to ABA and methyl jasmonate (MeJA) hut not to stress treatments. Possible functions of BnLea3-1 and another member of the Group 3 Lea family BnLea3-2 in embryo development is discussed.
Datla Raju
Huang Jun
Selvaraj Gopalan
Koenig Hans
National Research Council Of Canada
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