Promoters of the genes glutamate deshydrogenase,...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/80 (2006.01) C07K 14/37 (2006.01) C07K 19/00 (2006.01) C12N 1/15 (2006.01) C12N 9/06 (2006.01) C12N 9/24 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/53 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12P 1/02 (2006.01) C12P 21/02 (2006.01) C12P 35/00 (2006.01) C12P 37/00 (2006.01)

Patent

CA 2253250

The invention relates to promoters of the genes glutamate desydrogenase, .beta.-acetylhexosaminidase and .gamma.-actine and their use in systems of expression, secretion and anti-sens of filamentary funghi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate desydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) .gamma.-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) .gamma.-actine of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. Said promoters can also be used for blocking the genic expression through anti-sens construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and/or proteins inherent to the same.

L'invention concerne des promoteurs des gènes codant la glutamate déhsydrogénase, la .beta.-N-acétylhexoseminidase et la .gamma.-actine et leur utilisation dans des systèmes d'expression, de sécrétion et dans des constructions antisens de champignons filamenteux. L'invention concerne en outre l'utilisation des promoteurs des gènes codant: (I) la glutamate déshydrogénase NADP dépendant (EC.1.4.1.4) du champignon Penicillium chrysogenum, (II) la -N-acétylhexoseminidase (EC.3.2.1.52) du champignon Penicillium chrysogenum et (III) la .gamma.-actine de Penicillium chrysogenum, lesquels peuvent être utilisés pour la construction de puissants vecteurs d'expression et de sécrétion qui sont utiles pour le P. chrysogenum, pour A. chrysogenum et pour des espèces associées. De même, ces promoteurs peuvent être utilisés pour bloquer l'expression génétique par le biais de constructions antisens. Par l'intermédiaire des promoteurs susmentionnés, il est possible de promouvoir l'expression d'autres gènes dans des champignons filamenteux, ce qui a pour effet d'augmenter la production d'antibiotiques et/ou de protéines inhérentes à ceux-ci.

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