Proofreading primer extension

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2733903

The present invention provides for primer extension reactions, including polymerase chain reactions, in which a polymerase having 3' -5' exonuclease activity edits a primer that is not fully complementary thereby allowing for amplification and detection of target nucleic acids that may have variability in their sequences.

La présente invention porte sur des réactions d'extension d'amorce, comprenant des réactions en chaîne par polymérase, dans lesquelles une polymérase ayant une activité exonucléase 3' ou 5' édite une amorce qui n'est pas entièrement complémentaire, permettant ainsi une amplification et une détection des acides nucléiques cibles qui peuvent présenter une variabilité dans leurs séquences.

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