Propagation process

C - Chemistry – Metallurgy – 12 – N

Patent

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47/4, 195/47, 19

C12N 5/00 (2006.01) A01G 7/00 (2006.01) A01H 1/02 (2006.01) A01H 4/00 (2006.01) C12N 5/04 (2006.01) C12N 5/14 (2006.01)

Patent

CA 1262239

Propagation Process Abstract of the Disclosure The invention describes a process for culturing proliferating aggregates of plant cells, which comprises a) uniformly plating isolated protoplasts, or isolated cells regenerated from proto- plasts, in or on an agarose-solidified culture medium, and/or b) cutting this pretreated and solidified culture medium into segments, transferring said segments to a liquid nutrient solution and continuing culturing in both cases until the cell aggregates have attained the desired size.

452955

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