Protamine-reactive igm antibodies

C - Chemistry – Metallurgy – 12 – N

Patent

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167/37, 530/15.0

C12N 15/02 (2006.01) A61K 39/395 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1340982

The present invention is directed to protamine-reactive, IgM antibodies, and their uses in prognosis, diagnosis, and therapy. In a specific embodiment, the invention relates to low affinity binding, protamine-reactive serum IgM antibodies. In particular, such antibodies can recognize a sequence comprising four arginyl residues, including a triplet, within a six amino acid residue sequence. Such antibodies may be natural antibodies (i.e., not induced). A low affinity subset of serum protamine-reactive IgM antibodies may be assayed for prognosis or diagnosis of AIDS. Such antibodies are detectable in sera of normal subjects and HIV-infected individuals who subsequently exhibit a significant period of latency, but are absent or deficient in sera of individuals diagnosed with AIDS and sera of HIV infected individuals, who though asymptomatic at the time of the sampling, proceed to AIDS within a relatively short time. The invention provides methods and diagnostic kits for the detection and/or measurement of the low affinity subset of protamine-reactive serum IgM antibodies, for the prognosis and diagnosis of AIDS and other immune system abnormalities.

594360

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