Protease

Details Protease Protease

C - Chemistry, Metallurgy

12

N

195/128, 195/39,

C12N 9/58 (2006.01) C12N 9/50 (2006.01) C12N 9/60 (2006.01) C12P 21/06 (2006.01)

Patent

CA 1318872

Abstract There is disclosed a protease having the following enzymatic properties in view of: (a) function and substrate specificity, (b) optimum pH (f) activation (g) inhibition (a) it can hydrolyze, in particular, a peptide bond on the C-terminal side of Y of a compound X-Y-, in which X is Arg, Lys or Pro, whether or not to have a peptide bond on the N-terminal side, Y is Arg and - indicates a peptide bond; (b) about 7.0 in Tris hydrochloride buffer; (f) it is activated with calcium chloride or a surfactant; (g) it is inhibited with p-amidinophenylmethanesulfonyl fluoride, p-chloromercuribenzoic acid, a metal chelater, tetraacetic acid or a heavy metal.

589189

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