Protease

Details Protease Protease

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) A61K 38/48 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/50 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2360585

The invention relates to protease with two aspartate radicals in a catalytically active structure, a first aspartate radical being in a sequence X1GX2GD and a second aspartate radical being in a sequence X3X4DX5, X1, X2, X3 and X5 being selected independently of each other from the following: Ala, Val, Leu, Met and Ile; and X4 being an aromatic amino acid. The sequences X1GX2GD and X3X4DX5 lie in a transmembrane region.

L'invention concerne une protéase comportant deux restes aspartate dans une structure catalytiquement active, un premier reste aspartate se situant dans un motif X¿1?GX¿2?GD et un deuxième reste aspartate se situant dans un motif X¿3?X¿4?DX¿5?. La protéase selon l'invention est caractérisée en ce que X¿1?, X¿2?, X¿3? et X¿5? pouvent être choisis indépendamment les uns des autres parmi Ala, Val, Leu, Met et Ile, en ce que X¿4? est un acide aminé aromatique et en ce que les motifs X¿1?GX¿2?GD et X¿3?X¿4?DX¿5? se situent dans une région transmembranaire.

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