Protease deficient caulobacter host cells

Details Protease deficient caulobacter host cells Protease deficient caulobacter host cells

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C12N 15/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2447797

Caulobacter optimized for use in expression of heterologous peptides as part of a hybrid Caulobacter S-layer protein are provided as well as methods for producing such Caulobacter. Caulobacter of this invention are deficient in a native Caulobacter protease that cleaves hybrid S-layer proteins. Also provided are Caulobacter libraries and methods for sorting such libraries using cell sorting technology such as FACS.

L'invention concerne des bactéries Caulobacter optimisées pour l'expression de peptides hétérologues faisant partie d'une protéine hybride à couche de surface de Caulobacter, ainsi que des procédés de production de ces Caulobacter. CesCaulobacter présentent une déficience en une protéase native de Caulobacter clivant des protéines hybrides à couche de surface. L'invention concerne également des bibliothèques de Caulobacter et des procédés pour trier ces bibliothèques par des techniques de triage de cellules, telles que la technique FACS.

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