Protease detection

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 3/00 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2705432

A polypeptide comprising a chromogenic amino acid. The chromogenic amino acid is flanked by at least one amino acid to the N and C termini thereof. The amine group of the chromogenic amino acid has a pKa of less than 5. The chromogenic amino acid is capable of reacting with a conjugated aldehyde. The polypeptide comprises a target sequence for a target protease which is capable of cleaving the peptide bond comprising the amino group of the chromogenic amino acid.

L'invention porte sur un polypeptide comportant un acide aminé chromogène. L'acide aminé chromogène est encadré par au moins un acide aminé aux extrémités terminales N et C de celui-ci. Le groupe amine de l'acide aminé chromogène a un pKa de moins de 5. L'acide aminé chromogène est capable de réagir avec un aldéhyde conjugué. Le polypeptide comporte une séquence cible pour une protéase cible qui est capable de cliver la liaison peptidique comportant le groupe amino de l'acide aminé chromogène.

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