C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/57 (2006.01) C11D 3/386 (2006.01) C12N 9/54 (2006.01)
Patent
CA 2293695
The present invention relates to cleaning compositions comprising protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position 210 in combination with one or more acid residue positions equivalent to those selected from the group consisting of: +33, +62, +67, +76, +100, +101, +103, +104, +107, +128, +129, +130, +132, +135, +156, +158, +164, +166, +167, +170, +209, +215, +217, +218 and +222, where the numbered positions correspond to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin).
Cette invention se rapporte à des compositions nettoyantes, comprenant une enzyme protéase constituée par une variante de carbonyle hydrolase ayant une séquence d'acides aminés ne se trouvant pas à l'état naturel, qui est dérivée par remplacement de plusieurs restes d'acides aminés d'une carbonyle hydrolase précurseur par différents acides aminés. Ces restes d'acides aminés remplacés dans l'enzyme précurseur correspondent à la position +210 combinée aux positions d'un ou de plusieurs restes d'acides aminés équivalentes à celles choisies dans le groupe +33, +62, +67, +76, +100, +101, +103, +104, +107, +128, +129, +130, +132, +135, +156, +158, +164, +166, +167, +170, +209, +215, +217, +218 et +222, les positions ainsi numérotées correspondant à la subtilisine existant à l'état naturel provenant du Bacillus amyloliquefaciens ou à des restes d'acides aminés équivalents d'autre carbonyles hydrolases ou subtilisines, telles que la subtilisine du Bacillus lentus.
Bott Richard Ray
Correa Paul Elliott
Graycar Thomas Paul
Rai Saroj
Zhu Yong
Dimock Stratton Llp
Genencor International Inc.
The Procter & Gamble Company
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