Protease, reverse transcriptase and endonuclease of...

C - Chemistry – Metallurgy – 12 – N

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195/39, 195/1.33

C12N 15/62 (2006.01) C07K 14/15 (2006.01) C07K 14/16 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01) C12N 9/22 (2006.01) C12N 9/50 (2006.01) C12N 15/54 (2006.01) C12N 15/55 (2006.01) C12P 21/06 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2004646

ABSTRACT Disclosed is a method for producing retroviral proteins which are protease, reverse transcriptase, endounuclease and Gag proteins. The method is characterized by the consecutive accomplishment of expression and processing of retroviral genes and these products under the stepwise cultivation of hosts transformed with a vector constructed to carry retroviral gene fragments comprising at least a protease gene and one or more of the other genes coding for retroviral proteins. The retroviral proteins of this invention is used as specific reagens for the diagnosis of retroviral disease, e.g., AIDS, malignant tumors and so forth, also may be used as the basis for research and development of antiviral agents and a vaccine against the above infectious diseases, and for genetic engineering.

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