Protease screening methods and proteases identified thereby

Details Protease screening methods and proteases identified thereby Protease screening methods and proteases identified thereby

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) C12N 9/50 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2656531

Methods for identifying modified proteases with modified substrate specificity or other properties are provided. The methods screen candidate and modified proteases by contacting them with a substrate, such as a serpin, an alpha macro globulins or a p35 family protein or modified serpins and modified p35 family members or modified alpha macroglobulins, that, upon cleavage of the substrate, traps the protease by forming a stable complex. Also provided are modified proteases.

L'invention concerne des procédés pour identifier des protéases modifiées avec une spécificité de substrat modifiée ou autres propriétés. Les procédés permettent de cribler des protéases candidates et modifiées par mise en contact de celles-ci avec un substrat, tel qu'une serpine, des macroglobulines alpha ou une protéine de la famille p35 ou des serpines modifiées et des membres modifiés de la famille p35 ou des macroglobulines alpha modifiées, qui, lors du clivage du substrat, piège la protéase par la formation d'un complexe stable. L'invention concerne également des protéases modifiées.

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