Protected binding assay

Details Protected binding assay Protected binding assay

G - Physics

01

N

167/44

G01N 33/543 (2006.01) G01N 33/544 (2006.01) G01N 33/566 (2006.01) G01N 33/78 (2006.01)

Patent

CA 1231049

ABSTRACT A specific binding enzyme-resistant ligand assay test material is provided, which material comprises (a) a solid phase incorporated with one partner of a specific binding pair com- prising said ligand or a binding analog thereof and a specific binding protein therefor; (b) a conjugate comprising the other partner of said specific binding pair incorporated with a sub- stance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme. The invention further pro- vides a specific binding method of assaying for an enzyme- resistant ligand in a sample, which method uses the above test material.

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