Protein arrays for allelic variants and uses thereof

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 40/10 (2006.01) C07K 1/04 (2006.01) C07K 14/47 (2006.01) C07K 17/00 (2006.01) C12N 9/02 (2006.01) C12N 11/00 (2006.01) C12Q 1/26 (2006.01) C40B 50/06 (2006.01) G01N 33/543 (2006.01) G01N 33/573 (2006.01) G01N 33/68 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2469403

The invention describe protein arrays and their use to assay, in a parallel fashion, the protein products of highly homologous or related DNA coding sequences. By highly homologous or related it is meant those DNA coding sequences which share a common sequence and which differ only by one or more naturally occurring mutations such as single nucleotide polymorphisms, deletions or insertions, or those sequences which are considered to be haplotypes (a haplotype being a combination of variations or mutations on a chromosome, usually within the context of a particular gene). Such highly homologous or related DNA coding sequences are generally naturally occurring variants of the same gene. Arrays according to the invention have multiple for example, two or more, individual proteins deposited in a spatially defined pattern on a surface in a form whereby the properties, for example the activity or function of the proteins can be investigated or assayed in parallel by interrogation of the array.

L'invention concerne des réseaux de protéines et l'utilisation de ceux-ci pour tester en parallèle des produits protéiques de séquences de codage d'ADN hautement homologues ou relatives. Hautement homologues ou relatives signifie que ces séquences de codage d'ADN partagent une séquence commune et diffèrent seulement d'une ou de plusieurs mutations d'origine naturelle, telles que des polymorphismes nucléotidiques uniques, des délétions ou des introductions, ou que ces séquences sont considérées comme étant des haplotypes (soit une combinaison de variations ou de mutations sur un chromosome, généralement pour un gène particulier). Ces séquences de codage d'ADN hautement homologues ou relatives sont généralement des variants du même gène d'origine naturelle. Des réseaux selon l'invention présentant plusieurs, par exemple, au moins deux, protéines individuelles déposées, selon un motif défini dans l'espace, sur une surface, sous une forme permettant d'effectuer des recherches relatives aux propriétés, par exemple, l'activité ou une fonction des protéines, ou de tester celles-ci en parallèle, par interrogation du réseau,.

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