Protein assay by silver binding

Details Protein assay by silver binding Protein assay by silver binding

G - Physics

01

N

150/14

G01N 33/68 (2006.01)

Patent

CA 1225317

PROTEIN ASSAY BY SILVER BINDING ABSTRACT OF THE DISCLOSURE A novel, inexpensive, highly sensitive and quantitative assay for measuring protein in solution based on the capacity of protein to bind silver is des- cribed. In this procedure, protein samples are first treated with glutaraldehyde and then exposed to ammonia- cal silver. After a specified time, the reaction is terminated by the addition of sodium thiosulfate and the optical density measured at 420 nm. The useful range of the assay for the majority of standard proteins tested lies between 15 and 2000 ng. This represents a 100-fold increase in sensitivity over the coomassie Brilliant Blue dye binding procedure. There is little or no interference from carbohydrates, non-ionic detergents or ethanol. Pretreatment of protein samples with Bio-Gel P-2 to remove salts, thiol agents, EDTA and SDS, makes this procedure compatible with most commonly used buf- fers.

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