Protein complexes having factor viii:c activity and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A61K 38/37 (2006.01) C07K 7/06 (2006.01) C07K 14/755 (2006.01) C07K 14/81 (2006.01) C07K 16/00 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2068728

2068728 9107490 PCTABS00005 Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an .alpha.1-antitrypsin signal sequence.

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