C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/12 (2006.01) A61K 38/10 (2006.01) A61K 38/45 (2006.01) C07K 7/08 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2530667
The present invention relates to a protein crystal comprising the processivity clamp factor of DNA polymerase and a peptide of about 3 to about 30 amino acids, in particular of about 16 amino acids, said peptide comprising all or part of the processivity clamp factor binding sequence of a processivity clamp factor interacting protein, such as prokaryotic Pol I, Pol II, Pol III, Pol IV, Pol V, MutS, ligase I, .alpha. subunit of DNA polymerase, UmuD or UmuD', or eukaryotic pol .epsilon., pol .delta., pol .eta., pol .iota., pol .kappa..
La présente invention se rapporte à un cristal protéique présentant le facteur de pince de processivité de l'ADN polymérase et un peptide formé d'environ 3 à 30 acides aminés, en particulier d'environ 16 acides aminés, ledit peptide contenant tout ou partie de la séquence de liaison du facteur de pince de processivité d'une protéine interagissant avec le facteur de pince de processivité, telle que Pol I, Pol II, Pol III, Pol IV, Pol V procaryotes, MutS, ligase I, sous-unité a de l'ADN polymérase, UmuD ou UmuD', ou pol e, pol d, pol ?, pol ?, pol ? eucaryotes.
Burnouf Dominique Yves Joel
Dumas Philippe
Fuchs Robert Pierre Paul
Fujii Shingo
Olieric Vincent
Centre National de La Recherche Scientifique
Goudreau Gage Dubuc
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