C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) A61K 38/22 (2006.01) A61K 39/00 (2006.01) C07K 14/575 (2006.01) C07K 14/59 (2006.01) C12N 15/67 (2006.01) C12N 15/866 (2006.01)
Patent
CA 2635158
The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 x 10 5 to 5 x 10 6 cells/ml with an m.o.l of < 0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or B m8 protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 µg/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, .alpha.-units and/or .beta.- units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 µ/ml.
de Smit Abraham Johannes
Hamann Erik Kees
Kretzdorn Dietmar
Moormann Robertus Jacobus Maria
Van de Wiel Dirk Franciscus Marinus
Aktiengesellschaft Bayer
Borden Ladner Gervais Llp
Stichting Instituut Voor Dierhouderij En Diergezondheid
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