C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) A23K 1/17 (2006.01) A23L 1/305 (2006.01) C07K 14/415 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2617052
This invention is related to the extraction of a protein from the seeds, cotyledons or plantlets of Lupinus genus, as well as to the way of producing it in recombinant form and of expressing it in genetically modified plants. Due to the exceptional characteristics exhibited by this protein in what concerns: its potent antifungal and anti-Oomycete activity, which confers great potential to the protein as a fungicide, (2) its strong plant growth promoter activity, particularly notorious on unhealthy or naturally weakened plants, (3) its extreme resistance to denaturation, which allows the use of the protein under field conditions, (4) its great susceptibility to proteolytic attack, which makes it harmless to the environment and nontoxic for man, and (5) its well balanced amino acid composition. It is claimed its use, or of any modification of the protein that maintains its biological properties, as a supplement in human or animal nutrition and as a fungicide, insecticide, growth promoter, fertilizer or in the preparation of genetically modified organisms.
L'invention concerne l'extraction d'une protéine à partir de graines, de cotylédons ou de plantules du genre Lupinis, ainsi qu'un procédé de production de cette protéine sous forme recombinée et de son expression dans des plantes génétiquement modifiées. En raison des caractéristiques exceptionnelles de cette protéine: (1) activité antifongique et anti-oomycète puissante, ce qui confère à la protéine un pouvoir fongicide important; (2) activité puissante comme facteur de croissance des plantes, en particulier sur des plantes connues, malades ou naturellement fragilisées; (3) extrême résistance à la dénaturation, ce qui permet d'utiliser la protéine dans des conditions naturelles; (4) grande sensibilité aux attaques protéolytiques, ce qui la rend inoffensive pour l'environnement et non toxique pour l'homme; et enfin, (5) composition en aminoacides bien équilibrée; l'invention revendique l'utilisation de cette protéine, ou toute modification qui préserve ses propriétés biologiques, comme complément alimentaire humain ou animal ainsi que comme fongicide, insecticide, facteur de croissance, fertilisant ou dans la préparation d'organismes génétiquement modifiés.
Borges Loureiro Virgilio
de Seixas Boavida Ferreira Ricardo Manuel
Nascimento Teixeira Artur Ricardo
Valadas Da Silva Monteiro Sara Alexandra
Borges Loureiro Virgilio
Bull Housser & Tupper Llp
de Seixas Boavida Ferreira Ricardo Manuel
Instituto Superior de Agronomia
Nascimento Teixeira Artur Ricardo
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