Protein folding

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/113 (2006.01) C07K 14/495 (2006.01) C07K 14/51 (2006.01)

Patent

CA 2645109

The present invention concerns a method for folding a Transforming Growth Factor Beta, or a functional analogue thereof, into a dimeric, biologically active form. The method involves adding solubilized, unfolded monomeric growth factor to a solution containing 2-(cylcohexylamino)-ethanesulfonic acid (CHES) or a functional analogue thereof and a low molecular weight sulfhydryl/disulfide redox system. The solution is then incubated under conditions suitable for generating dimeric biologically active Transforming Growth Factor Beta.

L'invention concerne un procédé de repliement d'un facteur de croissance transformant bêta (TGF-.beta.), ou un analogue fonctionnel de celui-ci, en une forme dimère biologiquement active. Le procédé consiste d'abord à introduire un facteur de croissance monomère déplié et solubilisé dans une solution contenant de l'acide 2-(cylcohexylamino)-éthanesulfonique (CHES), ou un analogue fonctionnel de celui-ci, ainsi qu'un système redox de sulfhydryle/disulfure de faible poids moléculaire. Le procédé consiste ensuite à incuber la solution dans des conditions appropriées pour obtenir un facteur de croissance transformant bêta (TGF-.beta.) dimère biologiquement actif.

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