Protein for blocking platelet adhesion

Details Protein for blocking platelet adhesion Protein for blocking platelet adhesion

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2367457

A naturally occurring protein isolated from the saliva of the medicinal leech Hirudo medicinalis is described which strongly binds to collagen thus acting as an inhibitor of natural platelet adhesion to collagen. The protein has a molecular weight of about 12 000, an acidic isoelectric point and contains six cysteins. The protein was sequenced and the gene was cloned from a H. medicinalis cDNA-library. Procedures for producing such polypeptide by recombinant techniques are disclosed. The recombinant and the natural occurring proteins are potent inhibitors of collagen-dependent platelet adhesion and therefore useful for the therapeutic treatment of various conditions related to hard disease and diseases of the circulation system. Furthermore the protein is useful for coating natural or artificial collagen surfaces in order to render them nonadhesive for cells and prevent the activation of cells.

L'invention concerne une protéine naturelle isolée de la salive de la sangsue médicinale Hirudo medicinalis. Ladite protéine est fortement liée au collagène agissant ainsi comme inhibiteur de l'adhésion naturelle des plaquettes au collagène. La protéine a un poids moléculaire d'environ 12 000 et un point iso-électrique acide; elle contient 6 cystéines. La protéine a été séquencée et le gène a été cloné à partir d'une banque d'ADNc de H. medicinalis. L'invention décrit également les procédés de production de tels polypeptides par des techniques recombinantes. Les protéines naturelles et recombinantes sont des inhibiteurs efficaces de l'adhésion des plaquettes en fonction du collagène et sont donc utiles dans le traitement de différents états liés à des maladies graves et à des troubles de l'appareil circulatoire. De plus, la protéine est utile pour recouvrir les surfaces de collagène naturel ou artificiel en vue de les rendre non adhésives aux cellules et de prévenir ainsi l'activation des cellules.

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