C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 9/24 (2006.01) C12N 15/56 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01) C12N 15/62 (2006.01)
Patent
CA 2411968
The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an .alpha.-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoprotiens with reduced glycosylation using such genetically engineered stains of Pichia are also provided.
L'invention concerne des souches génétiquement modifiées de Pichia pouvant produire des protéines à glycosylation réduite. En particulier, ces souches génétiquement modifiées peuvent exprimer une .alpha.-1,2-mannosidase et/ou glucosidase II. Selon l'invention, ces souches peuvent être également modifiées pour que le gène OCH1 soit rompu. L'invention concerne également des procédés destinés à produire des glycoprotéines à glycosylation réduite par l'utilisation de ces souches génétiquement modifiées de Pichia.
C. J. Geysens Steven
Callewaert Nico L. M.
Contreras Roland
Flanders Interuniversity Institute For Biotechnology (vib)
Mcfadden Fincham
Research Corporation Technologies Inc.
Vib Vzw
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