Protein kinase c

Details Protein kinase c Protein kinase c

G - Physics

01

N

G01N 33/573 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2320787

A method of determining whether a protein kinase C (PKC) has been activated, the method comprising determining whether or to what extent a phosphorylatable site on the said protein kinase C, which site is associated with activation of said protein kinase C, has been phosphorylated. A reagent which is capable of distinguishing between the presence or absence of a phosphate moiety at a phosphorylatable site in a protein kinase C, which site is associated with activation of said protein kinase C. Preferably, the reagent is an antibody.

L'invention concerne un procédé permettant de déterminer si une protéine kinase C (PKC) a été activée. Ce procédé consiste à déterminer si ou dans quelle mesure un site phosphorylable présent sur cette protéine kinase C et associé à l'activation de cette dernière a été phosphorylé. L'invention concerne également un réactif capable de déterminer la présence ou l'absence d'une fraction phosphate sur le site phosphorylable d'une protéine kinase C, ce site étant associé à l'activation de cette protéine kinase C. Ce réactif est de préférence un anticorps.

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