Protein labelling with o6-alkylguanine-dna alkyltransferase

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) C12N 9/10 (2006.01) C12N 9/14 (2006.01) C12N 15/62 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01) G01N 33/535 (2006.01)

Patent

CA 2501061

The present invention relates to methods of transferring a label from suitable substrates to O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins, to suitable fusion proteins, to suitable variants of AGT, and to novel labelled fusion proteins obtained. A protein of interest is incorporated into an AGT fusion protein, the AGT fusion protein is contacted with an AGT substrate carrying a label, and the AGT fusion protein is detected and/or manipulated using the label in a system designed for recognising and/or handling the label.

L'invention concerne des procédés de transfert de marqueur depuis des substrats appropriés vers des protéines de fusion O?6¿-alkylguanine-ADN alkyltransférase (AGT). Elle concerne également des protéines de fusion appropriées, des variants appropriés de l'AGT, et des protéines de fusion marquées. On incorpore une protéine visée dans une protéine de fusion AGT, puis la protéine de fusion AGT est mise en contact avec un substrat AGT portant un marqueur, et ladite protéine de fusion AGT est décelée et/ou manipulée au moyen du marqueur dans un système conçu pour reconnaître et/ou manipuler le marqueur.

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